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Pathology Research Core - Component Services |
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1. Histology and immunohistochemical staining |
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ASSOC. DIRECTOR
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Phil Carpenter, Associate Professor of Clinical Pathology
email: pmcarpen@uci.edu
phone: 714-456-6141
FAX: 714-456-5873 |
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SERVICES OFFERED |
Services provided by this component of the Core include routine histology (tissue trimming, embedding, sectioning, H&E staining, histochemical staining and immunohistochemical staining) and the retrieval of paraffin-embedded blocks of normal and diseased tissue (by diagnosis) from our archives. A detailed list is provided on the order form. |
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COMPLIANCE |
IRB or IACUC approval is required for all services
requiring the use of human or animal tissue, respectively. Service
requests will NOT be processed without the necessary compliance information
(provide copy of appropriate approval form to Dr. Carpenter; also
see COMPLIANCE guidelines). |
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COST TO INVESTIGATOR |
Re-charge rates are listed on the order form. |
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ORDERING SERVICES |
Select either the PDF or WORD version
of the Histology service order form, complete the form and fax (714-456-5873)
or email to Ms. Farah Akhtar.
Investigators may use our departmental courier service to send samples
to UCIMC, where the Histology unit is located. Processed samples
can also be returned by courier. To send samples via courier, email
the request form to Ms. Akhtar (specifying that samples will
follow via courier) and include a copy of the request form with the
samples. Pack the samples securely in a sturdy box. Tightly seal
any containers filled with formalin. Address the box to Pathology
Research Services Core, c/o of Ms. Farah Akhtar, Bldg. 10, UCIMC.
Be sure to specify that samples are to be returned by courier also.
Take the box to the courier pickup location in the Hitachi Bldg.
(Room 214) on the School of Medicine campus. Mr. Sutherland will
send you an email on the day that samples are sent back to the Hitachi
Bldg. drop by return courier.
Contact Dr. Carpenter with questions about the service. |
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2. Morphometry and image analysis of tissue sections |
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ASSOC DIRECTOR |
Phil Carpenter, Associate Professor of Clinical Pathology
email: pmcarpen@uci.edu
phone: 714-456-6141
FAX: 714-456-5873 |
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SERVICES OFFERED |
This component of the Core provides services required to quantify cellular elements or molecular signals detectable by microscopic evaluation of tissue sections. This includes determining the number and/or size of cellular organelles; measuring the intensity of immunostaining of individual cells in a tissue section, and counting the percentage of cells and/or nuclei that are immunostained with a particular antibody. These analyses can be performed on sections which were H&E stained or immunostained in the Histology component of the Core, or on sections prepared by individual investigators. |
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COMPLIANCE |
IRB or IACUC approval is required for all services
requiring the use of human or animal tissue, respectively. Service
requests will NOT be processed without the necessary compliance information
(provide copy of appropriate approval form to Dr. Carpenter; also
see COMPLIANCE guidelines). |
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COST TO INVESTIGATOR |
The re-charge rate for this service is $18 per hr of data acquisition. |
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ORDERING SERVICES |
Contact Dr. Carpenter directly to arrange services. |
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3. Laser-capture micro-dissection of tissue sections |
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ASSOC DIRECTOR |
Robert Edwards, Assistant Professor of Pathology-in-Residence
email: redwards@uci.edu
phone: 949-824-8576
FAX: 949-824-2160 |
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SERVICES OFFERED |
This component of the Core allows investigators to fractionate
cell populations from tissue sections. For example, normal tissue can
be purified from tumor tissue on tissue sections containing a mixture
of these cell types. The core facility, located in Hewitt Hall, contains
a Leica CM3050 cryostat and a Leica LS-AMD outfitted with fluorescent
optics (allowing the selective visualization and capture of immunostained
cells), with a minimum beam width of less than 1 µm.
This service can be performed on sections that were H&E stained in the Histology component of the Core, or on sections prepared by individual investigators.
For vendor info on instruments or Supplies:
Leica
Links to LCM Protocols:
Leica Sample Prep |
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COMPLIANCE |
IRB or IACUC approval is required for all services
requiring the use of human or animal tissue, respectively. Service
requests will NOT be processed without the necessary compliance information
(see order form and COMPLIANCE guidelines; provide copy of appropriate
approval form to Dr. Edwards). |
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COST TO INVESTIGATOR |
All users must first undergo a two hour orientation
on the instrument with Dr. Edwards. There is no fee for UCI investigators,
and $100 per hour for non-UCI affiliated users. All investigators
requiring the services of a surgical pathologist to perform the microdissection
will be charged $100 per hour of LCM time. Investigators must purchase
appropriate caps with laser-capture membrane (Arcturus), or foiled-coated
slides (Leica).
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ORDERING SERVICES |
Contact Dr. Edwards to arrange services. Users performing their own microdissections must receive training on the use of the LCM from Dr. Edwards. LCM time must be reserved in advance and each session must be recorded in the log book. |
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4. Analyte Determination (Clinical Pathology Services) |
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ASSOC.
DIRECTOR |
Jane Emerson, Clinical Professor of Pathology
email: jemerson@uci.edu
phone: 714-456-7557
FAX: 714-456-8272 |
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SERVICES OFFERED |
This component of the Core provides investigators
at UCI, other medical centers, and industry with the complete spectrum
of clinical pathology (laboratory medicine) services that are available
for patient care at UCIMC. Click HERE to
view a comprehensive menu of tests available at UCI. Contact Dr.
Emerson about tests not included in the current menu. |
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COMPLIANCE |
IRB approval is required for all services performed as part of a clinical research study at UCI. Service requests will NOT be processed without the necessary compliance information (provide copy of appropriate approval form to Dr. Emerson). |
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COST TO INVESTIGATOR |
Re-charge rates are determined on a case-by-case basis in consultation with Dr. Emerson or the UCI Clinical Research Finance Administration (for specimens processed as part of a UCI clinical research study). For more information on the UCI Clinical Research Finance Administration, UCI users can go here (UCIMC intranet site). |
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ORDERING SERVICES |
Contact Dr. Emerson directly to arrange services. |
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5. Tissue bank and procurement service (TBPS) |
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ASSOC DIRECTOR |
Navneet
Narula,
Associate Clinical Professor of Pathology
email: nnarula@uci.edu
phone: 714-456-6141
FAX: 714-456-5873 |
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SERVICES OFFERED |
The Core will bank remnants of tissue (both normal and tumor) resected from patients undergoing surgery at UCIMC. Remnant tissue is the residual portion of a surgical specimen following processing for diagnostic purposes (see Tissue Custody Policy). Signed consent from each patient will also allow us to obtain peripheral blood samples (serum and DNA) and clinical follow-up at 6 month intervals for 3 years. Investigators may request access to tissue and blood samples, as well as clinical data. Requests for large amounts of tissue or multiple samples, or access to relatively rare specimens will be reviewed by the Core’s Tissue Use Committee. |
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COMPLIANCE |
IRB approval is required for access to this tissue.
Service requests will NOT be processed without the necessary compliance
information (see order form and COMPLIANCE guidelines; provide copy
of appropriate approval form to Dr. Narula). Investigators are encouraged
to contact
Dr. Narula concerning
the
availability
of tissue prior to submitting projects for IRB approval. |
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COST TO INVESTIGATOR |
Re-charge rates are listed on the order form. |
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ORDERING SERVICES |
Select either the PDF or WORD version
of the Tissue Bank request form, complete the form and fax (714-456-5873)
or email to Jeff Kim, TBPS Clinical
Coordinator for the Tissue Bank. Contact Mr. Kim or Dr. Narula with
questions about
the service. |
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6. Genotyping (includes nucleic acid extraction from blood and tissue sections) |
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ASSOC DIRECTOR |
John Krolewski, Associate Professor of Pathology
email: jkrolews@uci.edu
phone: 949-824-4089
FAX: 949-824-2160 |
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SERVICES OFFERED |
Genotyping services are performed in the Diagnostic Molecular Pathology lab and include a variety of PCR and/or Southern blot based analyses of mutant or polymorphic alleles. The lab will work with investigators to identify genotype-based tests that may be of value in clinical treatment and cancer control studies. This service can also perform mRNA-based testing for occult cancer or residual disease. In addition, this component of the Core provides automated nucleic acid extraction from whole blood, paraffin-embedded or frozen tissue sections. |
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COMPLIANCE |
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information (provide copy of appropriate approval form to Dr. Krolewski). |
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COST TO INVESTIGATOR |
Nucleic acid extraction re-charge rates are listed on the order form. Re-charge rates are determined on a case-by-case basis in consultation with Dr. Krolewski or the UCI Clinical Research Finance Administration (for specimens processed as part of a UCI clinical research study). |
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ORDERING SERVICES |
Contact Dr. Krolewski to arrange specific genotyping analyses. For nucleic acid extraction services, select either the PDF or WORD version of the Nucleic acid service order form, complete the form and fax (949-824-4936) or email to Dr. Pamela Ward. Contact Dr. Krolewski with questions about the service. |
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Copyright © 2008
The Department of Pathology & Laboratory Medicine,
University of California. All rights reserved. |
